Molecular and morphometric identification of plant parasitic nematodes

Authors

  • Francesca De Luca Istituto per la Protezione delle Piante, Consiglio Nazionale delle Ricerche, Via Amendola 122/D 70126 Bari
  • Augusta Agostinelli Istituto per la Protezione delle Piante, Consiglio Nazionale delle Ricerche, Via Amendola 122/D 70126 Bari

Keywords:

D2-D3 expansion domains, Internal Transcribed Spacers, phylogeny, Xiphinema americanum-group

Abstract

During a nematode survey in vineyards of the Veneto region (Italy), several soil samples were collected. On morphological and morphometric studies, a population from Conegliano Veneto was preliminarily identified as X. rivesi Dalmasso 1969. To support the morphological diagnosis, a molecular characterization of X. rivesi from Italy using the D2-D3 expansion segments of the 28S rDNA and the ITS containing region was undertaken. PCR-RFLP analyses were also performed to determine species-specific patterns for the Italian population of the nematode. Neighbor-Joining, Minimum Evolution and Maximum Parsimony analyses were used to reconstruct phylogenetic relationships within X. rivesi and other Xiphinema species.

Downloads

Issue

Vol. 94 (2011)

Section

Articles

License

Copyright © CREA – Council for Agricultural Research and Economics, Research Centre for Plant Protection and Certification (CREA-DC). Papers accepted for publication become the copyright of the journal REDIA.
Requests for permission to reproduce or reuse the published material should be addressed to the Managing Editor.